This site is located on the territory of National Park “Rila”, Bulgaria. Parangalitsa has been a reserve since 1933. It is situated on part of the southwestern slopes of Rila mountain, covering 1,509 hectares. It contains some of the oldest pine tree forests in Europe with an average age of over 200 years. The site is a part of the network of bioecological stations of Forest Research Institute – BAS and research is focused on highly productive coniferous forests with unique biomass. In the main experimental plots, long-term complex studies were conducted during the period 1979 - 1998, a large-scale database was created and a number of significant scientific results were obtained. In the period 2000 - 2018, separate targeted studies were carried out - focused on some ecosystem processes, which allows the analysis and evaluation of the functioning of ecosystems to be don

The site is located next to Wettingen in the canton of Aargau, within a natural, mixed forest stand which mainly consists of 150 year-old beech (Fagus sylvatica) and 120-170 year-old spruce (Picea abies) trees. Among others, the site is equipped with two meteorological measurement stations; one is located within the forest stand, the second is co-located in the nearby open-field, allowing to measure the forest stand effect on micro-climate. The following parameters are being measured since 2013: Soil solution chemistry (lysimeters), TREENET, atmospheric deposition (throughfall and bulk deposition), circular vegetation plots, crown condition assessment, diameter and height measurements, foliar analyses, leaf area index (LAI), litterfall, manual circumference band measurements, Ozone visible injury assessment, permanent vegetation quadrats, phenological observations, sapflow measurements, soil matrix chemistry, tree core sampling, automated point dendrometer measurements, automatic circumference band measurements.

As the largest contiguous pine forest in Switzerland, the Pfyn forest in Canton Valais (46° 18' N, 7° 36' E, 615 m ASL) offers the best conditions for such measurements. In light of this, a WSL research team installed a long-term experiment of 20 years duration in the Pfyn forest. The average temperature here is 9.2°C, the yearly accumulated precipitation is 657 mm (average 1961-1990). The pines in the middle of the forest are about 100 years old and 10.8 m high. The test area has 876 trees covering 1.2 ha divided into 8 plots of 1'000 m2 each (Image 4). Between the months of April and October four of these plots are irrigated by a sprinkler system providing an additional 700 mm of water, annually. In the other four plots, the trees grow under natural, hence relatively dry conditions.

The Seine estuary is a megatidal estuary located in northern France. It is the third largest estuarine ecosystem in the country after the Gironde and Loire along the French Atlantic coast; its waters flow into the English Channel. The geographical zone of influence of the Seine estuary runs from just upstream of the Poses dam, at the limit of the tidal penetration into the estuary — 160 km upstream of Le Havre —to the eastern part of the Bay of Seine. It can be divided into three sections: the fresh water upstream section (125 km), the mixing zone characterized by varying salinity levels (35 km), and the marine downstream section under the influence of the Seine River. The megatidal regime generates a turbidity maximum in the mixing zone (middle estuary) between the marine and fluvial sections of the estuary. The Seine estuary is a typical estuarine ecosystem: highly stressed by natural fluctuations and anthropogenic pressures, and hosting a rich ecological system. The Seine valley and its estuary are of major economic importance for France, with the presence of two maritime ports. It’s watershed (79,000 km2) is the home to 17 million people, and accounts for 50 % of the river traffic in France, 40 % of the country's economic activity, and 30 % of its agricultural activities. In addition to the Greater Paris area (> 11 million inhabitants), which contributes heavily to the Seine estuary's upstream inputs, two other major river settlements — Rouen (500,000 inhabitants) and Le Havre (300,000 inhabitants) — are maritime ports of international importance. Due to economic development, the Seine estuary has been subjected to major hydrodynamic, ecological, biogeomorphological and biogeochemical changes. The importance of studying the effects of anthropogenic impacts on estuarine ecosystems has increased in the last decade, especially under the Water Framework Directive, which aims to achieve “good environmental status” of all European water bodies.

The LTER-site of Brasschaat is a 2 ha scientific zone in a first generation plantation (1929) of Scots pine (Pinus sylvestris L.) on former heathland located in a mixed coniferous/deciduous forest in the Campine plain of Flanders. The landscape is flat, with a gentle (0.3%) slope at a mean elevation of 16 m. To the South and East, the forest extends over 2 km before turning into rural, partially forested terrain. The site is equipped with a measuring tower and an intensive forest monitoring plot (ICP Forests level II). The site is also part of an ICOS monitoring site which is operated by the University of Antwerp in collaboration with the Research Institute for Nature and Forest. The air pollution characteristics at this site are typical for a suburban forest exposed to vehicle emission (NOx), which is strongly affecting the NO-NO2-O3 chemistry. The site is, additionally, located in the waste plume derived from stack emissions (SO2, NOx, black carbon) generated by the petrochemical refinery in Antwerp port. Although no important agricultural emission sources are present in close proximity to the site, ammonia levels are elevated when winds blow from the eastern wind sector (due to remote agricultural activities > 5 km). The monitoring of air pollutants will be continued with special focus on chemical and biological recovery of the forest ecosystem from historical elevated sulphur and nitrogen depositions. Also the research on the combined effects of ozone, nitrogen and climate change on forest ecosystem functioning (gross primary production) will be emphasized.

Designation: Ecosystem research and management under land-use and climate change. Climate: Mean annual precipitation 150-200mm between November and April; mean maximum summer temperature 34° C, mean minimum winter temperature 6° C. (Data avialable for 15-min interval meteorological measurements and daily summaries for rainfall, air and soil humidity and temperature, wind velocity and direction, from 1997 to the present.) Principal biomes: Semiarid shrub land (shrub-grass steppe), rocky and loessial watersheds, ephemeral streams. Vegetation is dominated by patch-forming dwarf shrubs (Noaea mucronata, Atractylis serratuloides and Thymelea hirsuta) with species-rich annual winter vegetation in the inter shrub (dominated by Stipa capensis and other grasses) and shrub patch understory (dominated by Anagallis arvensis and other forbs). As a result of drought there are also two new sub biomes: crust land and grass land. Management: Livestock grazing excluded in central watershed (20 ha) since 1987; restricted/controlled grazing by Bedouin sheep herds in surroundings; in the outer parts of the area native and exotic trees were planted in 1985-87 in contour dykes (‘shikhim’) on the slopes and in terraces in the riverbed. Research: 1. Long-term experiments (up to 20 years) for monitoring changes in abundance, diversity, species composition and distribution, and development of biological soil crusts (BSC), perennial plants (dwarf shrubs) and winter annuals in relation to rainfall, soil disturbance, patch distribution, and livestock grazing. 2. long term hydrological studies on rainfall-runoff relationship under natural and manipulation experiments. 3. Short-term experiments and surveys (1 to 5 years) A. for testing hypotheses about the detailed processes, mechanisms and interactions involved in the development, dynamics and stability of shrub- and BSC-dominated patches, their landscape mosaic patterns and their feed-back relationships with flows of materials through the landscape; B. for testing hypothesis on the effect of geodiversity on the stability of shrub land to extreme drought and understanding state changes in drylands. 4. Long term studies on cross scale-cross level interactions in natural and human modified landscape. 5. Network-related research including regional comparisons of herbaceous plant productivity in open rangeland along the Israeli North-South rainfall gradient, and global comparison of woody plant diversity effects on ecosystem functionality of most dryland regions of the world.

The ZA PYGAR covers the whole Garonne river basin up to the upper part of the Gironde estuary (La Reole station). Within this territory, research is structured around 4 main sites: the Pyrénées mountains, covering the upstream part of the Garonne river basin (PYRénées site), the farmland of the foothills of the Pyrénées (Vallées et Coteaux de Gascogne site), the Garonne river (Axe Fluvial Garonne site) and the basin of two tributaries of the Garonne river in the Massif Central moutains (Aveyron-Viaur site).

The Kinneret Limnological Laboratory (KLL) is situated at the ‘Sapir’ Site (Tabha) on the shores of Lake Kinneret (Sea of Galilee). The Lake Kinneret monitoring program conducted by KLL has been operating since Jan. 1969. The program has provided a unique database and information, due to the sampling resolution, accuracy of the measurements, and their continuity, it is used to make operational decisions and environmental policy. The monitoring program includes several stations around the lake and from numerous depths and includes fixed on-lake, high-resolution, and manual sampling of water samples analyzed in the laboratory. The Kinneret monitoring program has expanded in recent years and currently has four fixed on-lake and online sampling platforms, strategically placed, each with thermistor chains and multi-probe sensor systems including one that is a profiling system. Also, the program includes collaboration with the Ministry of Agriculture to carry out monitoring of fish and fisheries in the lake. As the lake suffers typically from two seasonal cyanobacteria blooms, (i.e., a winter Microcystis bloom and a summer N-fixing cyanobacteria bloom) routing monitoring of cyanobacteria biomass and toxins is conducted and adaptive to the extent of the bloom. This is carried out simultaneously with satellite imaging over the lake.

Historically, the role of the Galapagos Islands has been considered critical due to the evolutionary diversification induced by natural and historical isolation. The Galapagos National Park was established in 1959, based on its historical importance associated with Charles Darwin's voyage and its rare and endemic biodiversity, composed of unique vertebrate and plant communities (Rozzi et al. 2010). In 1978, the Galapagos Islands were declared a UNESCO World Heritage Site, a critical factor associated with this national park (Rozzi et al. 2010). The Galapagos Islands are a pristine area with local and regional climates influenced by the interaction of ocean currents and winds driven by the Inter-Tropical Convergence Zone; ITCZ (Conroy et al. 2008, Trueman and d'Ozouville 2010). The long-term programme includes forty natural aquatic ecosystems (lakes, ponds, lagoons and swamps) that will be sampled on the islands of San Cristobal, Santa Cruz, Isabela and Floreana, where possible along a lowland-highland gradient. The number of sampling points will vary according to environmental heterogeneity and the presence of a littoral region, among other factors.

Monitoring stations in Aukštaitija NP started functioning in 1993. Aukstaitija station (LT01) was founded in strict reserve zone of Aukstaitija national park in Ažvinčių old forest, Versminis river closed basin. The area of the basin is 101.5 ha. The lowest place is at 159.5 m, the highest - 188.6 m above sea level. Geographical co-ordinates of the basin are: longitude - 26*03*20* - 26*04*50*, latitude - 55*26*00* - 55*26*53*. The climate is characterised as average cold with high humidity and abundant precipitation. The average air temperature is 5.8 C, average precipitation - 682 mm. Length of vegetation period - 189 days. Side glavioacvalic accumulation forms with sand, gravel and stones are typical for river basin and in the western part of the basin, with the decrease of surface altitude transfers into fliuvioglacial terrace delta plain with fine sand, and at the source- into marsh accumulation forms with organic sediments. At the eastern side of the basin, which has higher altitudes, the typical for this basin glavioacvalic accumulation forms transfer into gravel sand. Carbonates start foaming at 45 cm depth (Gulbinas et al., 1996). The saturation of the river water with the oxygen is very weak. The average yearly oxygen concentration equals to 2.9 mg/l (minimal allowable concentration – 6 mg/l). The pH value of the water fluctuates from 7.10-7.75 mg/l, which indicates the alkalisation of the water. Concentration of hydro-carbonate ions varies in the intervals of 148-224 mg/l. Concentrations of sulphates and chlorides are very small. Calcium and magnesium ions are dominating in the media of cations. Concentration of natrium ions is insignificant – 2 mg/l 9DLK=120 mg/l), concentration of kalium ions reaches only tenths of mg/l (DLK=50 mg/l). According to the size of mineralisation the river water could be described as having average mineralisation levels. Concentrations of nitrogen materials (ammonium nitrogen, nitrates) are small, compared to maximum allowable concentrations (DLK) set for surface waters. Concentrations of phosphates are small. Silicium concentrations fluctuate from 2 mg/l to 9 mg/l (Čeponienė, 1997). Main ecosystems Coniferous multi-layered forest stands are prevailing in the basin, which are mainly pine trees with a mixture of spruce. Those stands usually have a second and third tree layer with abundant under layer vegetation. Spruce stands are also of complicated structure. The second and third spruce layer with abundant under layer vegetation are common. Remarkable amounts of territory are occupied by mixed conifer-deciduous or mixed conifer forests. Pure birch stands in more wet habitats in the vicinity of Versminis river source can also be found. According to the forest stand age they are old pure overmature or with accompanying forest stands of several age classes. It is mainly multi-aged and multi-layered over mature pine and spruce stands. In addition The study was based on monitoring data on crown defoliation of more than 3000 Scots pine (Pinus sylvestris L.) trees from 20 permanent observation stands (POS) annually obtained between 1994 and 2009 in Aukstaitija national park (ANP), located in the eastern part of Lithuania (Fig. 1). Over 16yr period mean values of crown defoliation of every sample pine stand were computed annually. Therefore the total number of treatments made N=320. These stands were classified according to stand maturity: 4 sapling stands (45 to 50 years – Table 1, section “Stand maturity”, group 1), 5 middle aged stands (61 – 80 years – group 2), 4 premature stands (81-100 years – group 3), 3 mature stands (101-120 years – group 4) and 4 over mature stands (> 121 years – group 5). Main characteristics of the considered stands are compiled in table 1. All stands represent the prevailing in Lithuania Pinetum vaccinio-myrtilosum forest type.