3 sites with 10 ha altogether; follow the long-term changes on sand steppe gaps of juniper-poplar forest in order to study the effect of fire

Black Sea site includes three areas: cape Kaliakra, cape Galata and Varna Bay, and Koketrays Sand bank (only for macrozoobenthos). They were chosen on the base of historical data availability and eutrophication/pollution impact. Kaliakra marine area is influenced by eutrophication due to mainly the Danube river inflow. The local anthropogenic impact in the region is insignificant. It is a nature reserve. Cape Galata and Varna Bay sites are under the indirect impact of Varna Bay current and the system Beloslav lake-Varna lake - a cascade introducing nutrients and pollutants of industrial (chemical industry), agricultural, sewage origin and nearby ports. Koketrays Sand bank is an unique benthic habitat. The coordinates of the selected areas are as follow: c. Kaliakra: 28.416667 43.366667 c. Galata: 27.933889 43.186111 Varna Bay: 28.166667 43.166667 Koketrays bank: 27.888889 42.635556

The site, located along the Tyrrhenian coast, north of Rome city, is a Special Protection Area (SPA) for migratory and nesting birds (Anatidae, Ardeidae). The wetland area is one of the last stretches of the Lazio wetlands which have been progressively reclaimed and cultivated during the last century. The flora and the fauna of the site is not only highly specialized but also highly endangered, with a relevant ecological value evidenced in previous studies. The site includes coastal dune habitats of European interest, as the annual vegetation of drift lines, the embryonic shifting dunes, the mobile dunes with Ammophila arenaria and the dune grasslands. There are also habitats related to wet and mud soils, as Mediterranean salt meadows and reed beds dominated by Phragmites australis.

This site is located on the territory of National Park “Rila”, Bulgaria. Parangalitsa has been a reserve since 1933. It is situated on part of the southwestern slopes of Rila mountain, covering 1,509 hectares. It contains some of the oldest pine tree forests in Europe with an average age of over 200 years. The site is a part of the network of bioecological stations of Forest Research Institute – BAS and research is focused on highly productive coniferous forests with unique biomass. In the main experimental plots, long-term complex studies were conducted during the period 1979 - 1998, a large-scale database was created and a number of significant scientific results were obtained. In the period 2000 - 2018, separate targeted studies were carried out - focused on some ecosystem processes, which allows the analysis and evaluation of the functioning of ecosystems to be don

S.C.I IT7228221 Foce Trigno-Marina di Petacciato (Campobasso) S.C.I. IT7140108 Punta Aderci-Punta della Penna S.C.I. IT7140109 Marina di Vasto The site includes 3 Natura 2000 sites along the central Adriatic coast, where the last fragments of natural vegetation zonation on sand coastal dunes occur (shore vegetation, embryo and mobile dune vegetation, annual Mediterranean grasses and wet dune slacks).

This LTER-site corresponds with a 0.25 ha intensive forest monitoring plot (ICP Forests Level II) including a 0.25 ha buffer zone. The site is located in a homogeneous stand of Corsican pine (Pinus nigra ssp. laricio var. Corsicana Loud.) planted in 1930 on former heathland and is part of Gewestbos Ravels, a landscape (about 1000 ha) with deciduous and coniferous forests, heathland, bogs and pastures in the northern part of the Campine ecoregion of Flanders. The terrain is gently sloping (1-2 %) and the soil is a well-drained sandy soil (Endogleyic Folic Brunic Albic Arenosol (Dystric)). Ground vegetation is dominated by ferns (Dryopteris dilatata and D. carthusiana) and Vaccinium myrtillus. After a storm in February 2007 felled several trees an understorey of Betula pubescens started to develop. Intensive monitoring was started in 1987 with the main objective to study the effects of air pollution on biogeochemical cycling and forest performance (growth, vitality). This yielded a number of long-term data series including on atmospheric deposition, air quality, soil and soil solution chemistry, tree mineral nutrition, ground vegetation and growth. Monitoring will be continued in the future as a statutory task of INBO.

SCI IT7222217 Foce Saccione-Bonifica Ramitelli (Campobasso) The site includes 1 Natura 2000 site along the central Adriatic coast, where the last fragments of natural vegetation zonation on sand coastal dunes occur (shore vegetation, embryo and mobile dune vegetation, annual Mediterranean grasses, costa dunes with Juniperus spp and wet dune slacks).

The site Klausen-Leopoldsdorf is part of the European Level II Forest Monitoring System in the frame of the International Co-operative Programme on Assessment and Monitoring of Air Pollution Effects on Forests (ICP Forests). The site was established in 1995 and is, since 2006, part of the LTER Austria network. It is situated in a homogenous 80 years-old managed beech stand about 20km west of Vienna. The LTER-CWN research area in Klausenleopoldsdorf is divided into 3 subplots: a measuring weir, a dendrometer/sapflow measuring facility and GHG-chambers for measuring the fluxes of CO2 and other relevant GHG´s. All 3 subplots were installed in 2019 to collect high-resolution data. The measuring weir is located at about 475m a.s.l and the other subplots (denrometer/sapflow, GHG measuring chambers) are located at about 510m a.s.l. The observed catchment of the measuring weir has an area of 46 hectares. The dominating soil types in the catchment are Planosoils and Stagnosols. The subplots are situated in the near vicinity of the ICP-forest plot.

Suserup Skov (19.2 ha) is an old growth temperate forest dominated by beech Fagus sylvatica, pedunculate oak Quercus robur, ash Fraxinus excelsior, wych elm Ulmus glabra, and black alder Alnus glutinosa, admixed with lime Tilia platyphyllos and sycamore maple Acer pseudoplatanus. KU/IGN-SNB, is a 19,2 ha old-growth beech-dominated mixed deciduous forests on western Zealand owned by Sorø Academy. The forest use dates back to 4200 BC. A conservation act was placed on the forest in 1925 for biological and recreational purposes but allowing a minimum odd felling until the act was updated in 1961 to a non-intervention forest. The forest is unique at European level in terms of nonintervention, and the long-term status as non-intervention forest has led to a unique steady state in biomass and accumulation of dead wood, thus serving as an important reference site for unmanaged temperate deciduous forest development. There are long-term data records of e.g. biomass, dead organic matter, soil, forest structure, flora and fungi starting from 1992. The platform is included in a series of paired managed and non-intervention forests for studies of forest structure, biodiversity and biogeochemistry in mature and old beech-dominated forests. Suserup Forest includes an ICP Forests level II plot (2001-). Upgrades: eLTER master sites: soil moisture content (TDR), remote sensing of growth and forest structure, LAI, NDVI (Lidar, camera), continuous plant phenology (web cam),and insect monitoring (optical sensors) as biodiversity measures, ambient air quality and air pollution.

Vestskoven is a 15 km2 ongoing afforestation area located 15 km west of central Copenhagen. Since 1967 arable land has successively been bought up for afforestation in order to establish a large forest area for outdoor activities and wood production. In 1998, afforestation chronosequences in oak (Quercus robur) and Norway spruce (Picea abies) were established as a research platform to quantify soil carbon dynamics, water recharge, nitrogen budgets and ground vegetation species diversity. The soil and grund vegetation species composition were resampled in the chronosequences after 13 years in 2011. Since 2003, an ICP-Forests Level II/Core plot with integrated monitoring under the UN Convention on Long-Range Transboundary Air Pollution (CLRTAP) is placed in an oak stand. Monitoring has developed over time. There is no time limit on the monitoring financed by the Ministry of Environment and Food in Denmark.