The Northeast US Shelf (NES) ecosystem spans the Middle Atlantic Bight and Gulf of Maine, is a large portion of the NW Atlantic Shelves Province, and is in a coastal biome. The waters over the NES provide an array of ecosystem services including fishing, energy development, shipping, waste disposal, recreation, and conservation. The NES ecosystem, like other productive temperate shelf ecosystems, is characterized by strong seasonality along with high levels of variability in physical forcing at timescales from days to decades, including climate impacts.

Large marine site located in the Kvarnerić area, around the sheltered coast and waters of the eastern part of the Lošinj and Cres archipelago. It is one of the most important feeding and breeding areas for bottlenose dolphins (Tursiops truncatus) in the Eastern Adriatic. Lithostratigraphic units represented on the coast are dolomites and post sedimentary diagenetic breccia (upper albian, lower cenomanian - K16, K12), rudist limestones (cenomanianmaastricht - K21-6). Soils on the coast are rockyground, anthropogenic soil on karst. The area was created after the transgression of the sea after the last glaciation; marine shelf; highly indented coastline. In July 2006 of preventive protection of a part of the Kvarnerić region for 3 years as a Special Marine Reserve (Cres-Lošinj Special Marine Reserve – CLSMR has been declared.

Aquitain's karsts observatory (Aquitaine, France) regroups different karst sites located on the edge of the Aquitain sedimentary basin. These karst watersheds range from 0.01 km² (Lascaux cave) and 1 km² (Cussac cave) up to 100 km² (Toulon springs) and the land use consists of forests and agriculture under modified oceanic climate. This observatory consists of various observation and experimentation sites like patrimonial caves and large karstic hydrosystem outlets.

The Site comprises the Coastal Alagoas State portion of the Marine Protected Area Coral Coast (APACC), starting at the mouth of the Meirin River (Maceió, geographical coordinates: 9 ° 32'51 "S and 35 ° 36'59" W), following the preamar meanline, in the general northeasterly direction, bypassing and including the mangroves in all their extension to the left margin of the Persinunga River, on the border between Alagoas and Pernambuco States, geographical coordinates: 8 ° 54'49.04 "S and 35 ° 9'10.13" W, and advancing towards the direction of the Atlantic Ocean to the limit of the 10m isobath, according to the bathymetric chart of the Brazilian Navy. In this section, we can find significant samples of the main ecosystemic features present in APACC: estuaries, beach formations, marine angiosperm meadows, coralline reefs, and beach rocks.

The separation line of the semi-enclosed northern basin from the rest of the Adriatic Sea is conventionally defined at the 100 m isobath, at about 43°20’N, from where the bottom depth moderately decreases up to the northern coast reaching the smallest average depth in the northernmost protrusion of the basin, the Gulf of Trieste (surface area 600 km2, volume 9.5 km3, maximal depth approx. 25 m). The oceanographic properties of Gulf of Trieste are affected by water mass exchange with the northern Adriatic at the open western boundary, by local meteorological conditions that induce a pronounced seasonal cycle of seawater temperature (6 - 28 oC) and by the largest freshwater input, the Soča (Isonzo) River. The latter enters the Gulf on the shallower northern coastline with an average flow of 123 m3 s-1 and represents the major source of land-borne nutrients in this coastal zone, in particular of nitrate, whereas inputs along the south-eastern coast are comparatively small (contributing less than 10% to the freshwater balance in the Gulf). The circulation pattern is variable, however the prevailing ENE wind is the most efficient for the water exchange, especially during winter. These physical features, in addition to freshwater inputs, are ultimately reflected in the distribution of nutrients and in the seasonal and inter-annual variability of plankton communities, primary production but also in events of hypoxia/anoxia and mucous macroaggregates, which have characterised the whole northern Adriatic basin in the recent past. The waters of the Gulf of Trieste have been monitored for several physical-chemical and biological variables since 70s’ but the longest and most continuous data set of biological variables is represented by the phytoplankton time series, which started in 1990 when the national monitoring programme has become operational (set by Slovenian Environmental Agency). From 2007 onwards, the monitoring programme complies with the regulations of the EU Water Framework Directive (2000/60/EC).

The site is divided into two different types of environments: 1. Coastal marine environments. Marine coastal areas of Sardinia, in particular, the Gulf of Asinara in the north and the Gulf of Olbia in the north-east, affected by different types and intensities of human impact and by the resulting presence of different environmental conditions from the point of view of water quality. 2. Lagoons and coastal ponds. Lagoons and coastal ponds located along the west-central coast of Sardinia, in the Gulf of Oristano. These sites are all important wetlands included in the Ramsar Convention, are IBA (Important Birds Area) and SIC and ZPS. The ownership of the sites is the Regional Government of Sardinia. Fisheries and aquaculture are generally the most important human activities, and there are also educational and recreational activities. They are affected by various environmental issues, mostly related to the process eutrophic.

The Transetto Senigallia-Susak is a transect of sampling stations located in the Northern Adriatic Sea. The IRBIM-CNR in Ancona and the Politechnical University of Marche collects from the end of years 80s physical, chemical and biological data. This is an excellent observing site to analyze the characteristics of water masses entering and leaving the North Adriatic Sea and their possible modification. In the site is also located the TeleSenigallia pylon.

UK ECN site. Windermere is in NW England in the English Lake District, an area of great natural beauty and a tourist destination since the 18th century. The dominant geological structure of the Lake District is a dome of Paleozoic rocks formed by uplift in the Tertiary producing a radial drainage pattern later enhanced during the Pleistocene glaciation, with major lakes occupying bedrock basins in steep-sided, flat-floored valleys. Windermere is the largest natural lake in England with a surface area of 14.8km2 and altitude of only 40m. The lake is divided by a shallow sill into two basins; North Basin (NB) (surface area c. 8km2, maximum depth 64m) and South Basin (SB) (surface area c. 6.7km2, maximum depth 42m). Windermere (ECN site (2o58'W 54o23'N)), has a catchment of 180km2 draining into the lake via two main rivers, several small tarns (lakes) and streams. The catchment is mainly uplands, grazed by sheep all year but also used intensively for recreational purposes. The valley villages are major tourist destinations with consequent increases in sewage input to the lake. Over the past 50 years levels of dissolved reactive phosphorus in the lake have more than doubled, reaching their highest levels in the 1980s. The effluent discharged into SB from the main sewage works is now P-stripped to reduce the nutrient loading to the lake. The lake is a Site of Special Scientific Interest (SSSI), a source of potable water, a major recreational facility and a specialised fishery (for charr, Salvelinus alpinus). The Freshwater Biological Association and latterly the Centre for Ecology & Hydrology have had a laboratory on the shore of Windermere for over 50 years resulting in a large body of scientific literature based on Lake District lakes.

The Elbe River is one of the major rivers in Central Europe. It springs at an altitude of about 1,400 meters in the Giant Mountains in the north of the Czech Republic. It flows through eastern, middle, and northern Bohemia, and its catchment area includes most of Bohemia. The Elbe traverses the northeastern part of Germany and flows into the North Sea. The total river length is 1,094 km. In the Czech Republic, the monitored river length is 371 km and the catchment area includes 49,933 km2. The average flow at the Czech-German borders is 311 m3 s-1. Many weirs dam the watercourse of Elbe River in the Czech Republic. This artificial intervention is crucial for the characteristics of the riverbed, its width, depth, and bottom substrate. The width of the riverbed in the monitored area ranges from approx. 10 m on the upper course to approx. 150 m on the lower course and river sections above the weirs. The depth of the river varies from 0.5 m in the upper parts and below the weirs to more than 5 m in the lower course and above the weirs. The bottom substrate varies from the stony, gravelly, and sandy fractions in shallow and fast-running sections to muddy/silty fractions in slow-running sections above the weirs. The main tributaries of the Elbe in Bohemia are Metuje, Orlice, Jizera, Ploučnice, Vltava, Ohře and Bílina rivers. The catchment area is densely populated. The river is exposed to various sources of pollution (organic, agricultural, and industrial) and hydromorphological pressures (straightening, shortening, regulation of meanders, and fragmentation of river continuity). There are seven permanent monitoring sites on the Elbe River in the Czech Republic: Děčín (river kilometer 747.9), Litoměřice (796.9), Obříství (842.1), Lysá nad Labem (878.8), Valy (954.7), Hradec Králové (994.4) and Vestřev (1051). The Elbe basin long term environmental research has been organised by the Ministry of Environment of the Czech Republic and cooperation institutions since 1993. More than 600 physicochemical parameters, including sensory properties of water, are regularly monitored in at least nine different types of matrices (water, sediment, periphyton, floats, sedimentable floats, benthos, fish tissues, fish fry, and Dreissena polymorpha tissue) and using passive samplers. Among the main monitored characteristics belong balances of macroelements (carbon, nitrogen, oxygen, phosphorus), concentrations of inorganic salts, heavy metals and various organic pollutants such as pesticides, personal care products (including drugs) and other anthropogenic substances in the environment. Characteristics such as abundance and species composition are regularly monitored in fish, macrophytes, macrozoobenthos, phytobenthos and plankton.

The Gulf of Venice is a gulf that borders modern-day Italy, Slovenia and Croatia, and is at the north of the Adriatic Sea between the delta of the Po river in northern Italy and the Istria peninsula in Croatia. The site is under a heavy antropic impact (nutrient loads, coastal towns, fishery, tourism maritime routes). In the past eutrophic events struck the ecosystem and, more recently, frequent mucus macro-aggregates extended in the whole basin. Study of long term historical time series of the biotic community in the Gulf of Venice in relation with the biogeochemical cycles and physical properties of sea-water have been conducted since the '70s.