Lake Candia is a small and shallow lake, located in North-Westerm Italy. The mean elevation is 226 m a.s.l., the surface is 1,5 km², the max depth 7,7 m, the mean depth 4,7 m and the volume 0,007 km3. Lake Candia has suffered during its evolution a natural increase of its trophic state, undergoing a process of eutrophication culminated with a marked worsening of his ecological quality since the '60s. In the late '70s and mid '80s, this ecosystem was affected by fish mortality episodes and by the enormous development of emerged and submerged aquatic macrophytes. The systematic scientific study of Lake Candia began in 1985, when the CNR of Verbania was asked to perform a limnological research with the aim to propose a possible restoration intervention. In addition to the control of pollution sources from the basin, environmentally sound technologies were applied to reduce the effects of eutrophication, such as biomanipulation of the aquatic food chain and macrophyte control and management.The experiment conducted in Lake Candia has shown that significant improvements can be achieved through the management of a specific key components of the biocenosis of the lake. Data collection activities on hydrochemistry, phytoplankton, zooplankton, fish fauna, macrophytes, as well as physical limnology, took place from 1986 until 2012. To date, only hydrometeorological data collection (precipitation, air temperature, wind, solar radiation and lake levels), started in 1987, is active. LTER specific topics are: Long-term analysis of changes in community functional diversity of phyto-and zooplankton. Evaluation of the lake ecosystem response to short and intense rainfall events, in relation to the increase of nutrients input through runoff.

The Transetto Senigallia-Susak is a transect of sampling stations located in the Northern Adriatic Sea. The IRBIM-CNR in Ancona and the Politechnical University of Marche collects from the end of years 80s physical, chemical and biological data. This is an excellent observing site to analyze the characteristics of water masses entering and leaving the North Adriatic Sea and their possible modification. In the site is also located the TeleSenigallia pylon.

The Gulf of Venice is a gulf that borders modern-day Italy, Slovenia and Croatia, and is at the north of the Adriatic Sea between the delta of the Po river in northern Italy and the Istria peninsula in Croatia. The site is under a heavy antropic impact (nutrient loads, coastal towns, fishery, tourism maritime routes). In the past eutrophic events struck the ecosystem and, more recently, frequent mucus macro-aggregates extended in the whole basin. Study of long term historical time series of the biotic community in the Gulf of Venice in relation with the biogeochemical cycles and physical properties of sea-water have been conducted since the '70s.

UK ECN Site. Esthwaite Water is a natural lake situated in a glacial valley and is generally agreed to be the most productive or eutrophic lake in the English Lake District. It lies approximately 65 m above sea level and has an area of 1 km2 and a maximum depth of 15.5 m. The average retention time is 90 days. The catchment area is 17.1 km 2 and the hills are composed geologically of Bannisdale slates and grits. The surrounding land is used chiefly for agricultural purposes and forestry. The lake is a grade 1 Site of Special Scientific Interest and has been a designated "Ramsar" site since November 1991. The diverse aquatic invertebrate fauna includes a number of species with restricted distributions in Britain, one of which is the flatworm, Bdellocephala punctata. The slender naiad, Najas flexilis, which is listed as Nationally Scarce, has been found in Esthwaite Tarn. Artificial enrichment of the lake occurs by input from the Hawkshead Sewage Treatment Works (which has operated a continuous programme of phosphate stripping since 1989) and originally from effluents from the fish farm which used to be situated towards the south of the lake. The lake undergoes summer stratification with oxygen depletion regularly below 7 m and sometimes as shallow as 5 m. The phytoplankton tends to be dominated by diatoms in spring and by cyanobacteria for much of the summer.

UK ECN site. Windermere is in NW England in the English Lake District, an area of great natural beauty and a tourist destination since the 18th century. The dominant geological structure of the Lake District is a dome of Paleozoic rocks formed by uplift in the Tertiary producing a radial drainage pattern later enhanced during the Pleistocene glaciation, with major lakes occupying bedrock basins in steep-sided, flat-floored valleys. Windermere is the largest natural lake in England with a surface area of 14.8km2 and altitude of only 40m. The lake is divided by a shallow sill into two basins; North Basin (NB) (surface area c. 8km2, maximum depth 64m) and South Basin (SB) (surface area c. 6.7km2, maximum depth 42m). Windermere (ECN site (2o58'W 54o23'N)), has a catchment of 180km2 draining into the lake via two main rivers, several small tarns (lakes) and streams. The catchment is mainly uplands, grazed by sheep all year but also used intensively for recreational purposes. The valley villages are major tourist destinations with consequent increases in sewage input to the lake. Over the past 50 years levels of dissolved reactive phosphorus in the lake have more than doubled, reaching their highest levels in the 1980s. The effluent discharged into SB from the main sewage works is now P-stripped to reduce the nutrient loading to the lake. The lake is a Site of Special Scientific Interest (SSSI), a source of potable water, a major recreational facility and a specialised fishery (for charr, Salvelinus alpinus). The Freshwater Biological Association and latterly the Centre for Ecology & Hydrology have had a laboratory on the shore of Windermere for over 50 years resulting in a large body of scientific literature based on Lake District lakes.

Lake Võrtsjärv is the largest inland water body in Estonia. Is located in Central part of the country (58.286560, 26.049585). Is a large (270 km2) shallow polymictic lake with a maximum depth of 6 m and a mean depth of 2.8 m. According to the classification by Lewis (1983) belongs to continuous rather than discontinuous cold polymictic lakes. Lake Võrtsjärv Buoy was deployed in the summer of 2010 near the deepest point of the lake (N 58.211798, E 26.103163) to gather high resolution data on meteorology and water quality of the lake. YSI EMM 700 bay buoy has been used for float. YSI 6600 V2-4 multisonde (at 1 meter) was fixed at 1 meter depth for underwater measurements. The buoy is equipped with a Vaisala Weather Transmitter WXT520 and a Li-Cor pyranometer (LI-200) for weather parameters. Data collection was controlled with Campbell CR1000 datalogger. System was renewed in 2021. Current underwater measurements are collected with EXO2 multisond and data collection is controlled by Flydog Marine datalogger.

The Elbe River is one of the major rivers in Central Europe. It springs at an altitude of about 1,400 meters in the Giant Mountains in the north of the Czech Republic. It flows through eastern, middle, and northern Bohemia, and its catchment area includes most of Bohemia. The Elbe traverses the northeastern part of Germany and flows into the North Sea. The total river length is 1,094 km. In the Czech Republic, the monitored river length is 371 km and the catchment area includes 49,933 km2. The average flow at the Czech-German borders is 311 m3 s-1. Many weirs dam the watercourse of Elbe River in the Czech Republic. This artificial intervention is crucial for the characteristics of the riverbed, its width, depth, and bottom substrate. The width of the riverbed in the monitored area ranges from approx. 10 m on the upper course to approx. 150 m on the lower course and river sections above the weirs. The depth of the river varies from 0.5 m in the upper parts and below the weirs to more than 5 m in the lower course and above the weirs. The bottom substrate varies from the stony, gravelly, and sandy fractions in shallow and fast-running sections to muddy/silty fractions in slow-running sections above the weirs. The main tributaries of the Elbe in Bohemia are Metuje, Orlice, Jizera, Ploučnice, Vltava, Ohře and Bílina rivers. The catchment area is densely populated. The river is exposed to various sources of pollution (organic, agricultural, and industrial) and hydromorphological pressures (straightening, shortening, regulation of meanders, and fragmentation of river continuity). There are seven permanent monitoring sites on the Elbe River in the Czech Republic: Děčín (river kilometer 747.9), Litoměřice (796.9), Obříství (842.1), Lysá nad Labem (878.8), Valy (954.7), Hradec Králové (994.4) and Vestřev (1051). The Elbe basin long term environmental research has been organised by the Ministry of Environment of the Czech Republic and cooperation institutions since 1993. More than 600 physicochemical parameters, including sensory properties of water, are regularly monitored in at least nine different types of matrices (water, sediment, periphyton, floats, sedimentable floats, benthos, fish tissues, fish fry, and Dreissena polymorpha tissue) and using passive samplers. Among the main monitored characteristics belong balances of macroelements (carbon, nitrogen, oxygen, phosphorus), concentrations of inorganic salts, heavy metals and various organic pollutants such as pesticides, personal care products (including drugs) and other anthropogenic substances in the environment. Characteristics such as abundance and species composition are regularly monitored in fish, macrophytes, macrozoobenthos, phytobenthos and plankton.

The separation line of the semi-enclosed northern basin from the rest of the Adriatic Sea is conventionally defined at the 100 m isobath, at about 43°20’N, from where the bottom depth moderately decreases up to the northern coast reaching the smallest average depth in the northernmost protrusion of the basin, the Gulf of Trieste (surface area 600 km2, volume 9.5 km3, maximal depth approx. 25 m). The oceanographic properties of Gulf of Trieste are affected by water mass exchange with the northern Adriatic at the open western boundary, by local meteorological conditions that induce a pronounced seasonal cycle of seawater temperature (6 - 28 oC) and by the largest freshwater input, the Soča (Isonzo) River. The latter enters the Gulf on the shallower northern coastline with an average flow of 123 m3 s-1 and represents the major source of land-borne nutrients in this coastal zone, in particular of nitrate, whereas inputs along the south-eastern coast are comparatively small (contributing less than 10% to the freshwater balance in the Gulf). The circulation pattern is variable, however the prevailing ENE wind is the most efficient for the water exchange, especially during winter. These physical features, in addition to freshwater inputs, are ultimately reflected in the distribution of nutrients and in the seasonal and inter-annual variability of plankton communities, primary production but also in events of hypoxia/anoxia and mucous macroaggregates, which have characterised the whole northern Adriatic basin in the recent past. The waters of the Gulf of Trieste have been monitored for several physical-chemical and biological variables since 70s’ but the longest and most continuous data set of biological variables is represented by the phytoplankton time series, which started in 1990 when the national monitoring programme has become operational (set by Slovenian Environmental Agency). From 2007 onwards, the monitoring programme complies with the regulations of the EU Water Framework Directive (2000/60/EC).

South-West Bulgaria, Mediterranean catchment

Western part of the Gulf of Riga (the Baltic Sea) littoral and sublitoral zone. The marine site is located on the west coast of the Gulf, in the Engure Nature protected area. The monitoring activities are based on three transects which represent an open area of very similar substrate type. These transects are located on the tip of Mersrags, 2.5 km northwards from the port of Mersrags. Long-term studies of the Gulf of Riga coastal zone communities and macrophytes as the key component of littoral zone under natural and anthropogenic impact and possible threats to the littoral zone biological resources. The SCUBA diving is used in marine site assessment.