The site, located along the Tyrrhenian coast, north of Rome city, is a Special Protection Area (SPA) for migratory and nesting birds (Anatidae, Ardeidae). The wetland area is one of the last stretches of the Lazio wetlands which have been progressively reclaimed and cultivated during the last century. The flora and the fauna of the site is not only highly specialized but also highly endangered, with a relevant ecological value evidenced in previous studies. The site includes coastal dune habitats of European interest, as the annual vegetation of drift lines, the embryonic shifting dunes, the mobile dunes with Ammophila arenaria and the dune grasslands. There are also habitats related to wet and mud soils, as Mediterranean salt meadows and reed beds dominated by Phragmites australis.

The site consists of high elevation vegetation in central Mediterranean basin, along the Apennines mountain range: xerophitic primary grassland (Pediculari elegantis-Seslerietum tenuifoliae) and mesophitic primary grassland (Luzulo italiacae-Festucetum macratherae), with important resident and migratory bird communities. Six plots, each one 100 m2 large, grouped in two cluster-plots including each one three plots, representative of a larger area of 0,5 km2 ca., ranging from 2100 to 2300 m a.s.l. (alpic and Mediterranean high-mountain belts.

The Hölstein site is located in in the eastern part of the Swiss Jura mountains (47°26’16.4’’, 7°46’31.1’’; 540 m a.s.l.), in a mixed, temperate forest with an exceptionally high biodiversity and a vigorous tree regeneration. The research plot has a size of 1.6 ha and comprises 540 mature trees. The main tree species are Fagus sylvatica and Picea abies with an admixture of Abies alba, Quercus petraea, Pinus sylvestris, Acer pseudoplatanus, Carpinus betulus, Fraxinus excelsior and Sorbus torminalis. A canopy crane is installed in the center of the research area to access the tree canopies for in situ measurements and for sampling of leaf material. Rainout shelters will be installed in 2019 for conducting a long-term throughfall exclusion experiment.

Mountain site, alpine forests Sierra Nevada (Andalusia, SE Spain), is a mountainous region with an altitudinal range between 860 m and 3482 m a.s.l. covering more than 2000 km2. The climate is Mediterranean, characterized by cold winters and hot summers, with pronounced summer drought (July-August). The annual average temperature decreases in altitude from 12-16ºC below 1500 m to 0ºC above 3000 m a.s.l., and the annual average precipitation is about 600 mm. Additionally, the complex orography of the mountains causes strong climatic contrasts between the sunny, dry south-facing slopes and the shaded, wetter north-facing slopes. Annual precipitation ranges from less than 250 mm in the lowest parts of the mountain range to more than 700 mm in the summit areas. Winter precipitation is mainly in the form of snow above 2000 m of altitude. The Sierra Nevada mountain range hosts a high number of endemic plant species (c. 80; Lorite et al. 2007) for a total of 2,100 species of vascular plants (25% and 20% of Spanish and European flora, respectively), being considered one of the most important biodiversity hotspots in the Mediterranean region. This mountain range has several legal protections: Biosphere Reserve MAB Committee UNESCO; Special Protection Area and Site of Community Importance (Natura 2000 network); and National Park. The area includes 61 municipalities with more than 90,000 inhabitants. The main economic activities are agriculture, tourism, cattle raising, beekeeping, mining, and skiing.

For over 20 years as a team of Long Term Sites in Serra do Cipó PELD-CRSC has been working to answer crucial questions of evolutionary ecology and provide a basis for understanding the spatio-temporal ecosystem functioning and it is future challenges with changes in climate and land use.

Berchtesgaden National Park was established in 1978 and is the only German national park located at the Alps. It comprises three major valleys and covers an elevantional range from 600 to 2700 m. Ecosystems include montane mixed forest and subalpine coniferous forest, alpine grasslands, pastures and rocky habitats. The Department for Research and Monitoring is linked to the Techincal University of Munich. It conducts monitoring on climate, hydrology, ecosystem dynamics and biodiversity. Main research topics include drivers of natural ecosystem dynamics, human impacts on ecosystems and climate change effects on ecosystems.

The Cairngorms National Park is one of 15 National Parks in the UK (one of two in Scotland) and is Britain's largest National Park (4,528 km2). It is the location for some of the most spectacular landscapes in Britain and is home to an incredible diversity of wildlife and plants. http://cairngorms.co.uk/the-park/facts-figures/ The aims of the National Park are: • To conserve and enhance the natural and cultural heritage of the area; • To promote sustainable use of the natural resources of the area; • To promote understanding and enjoyment (including enjoyment in the form of recreation) of the special qualities of the area by the public; • To promote sustainable economic and social development of the area’s communities. Cairngorms National Park, seven broad habitats can be identified: • Enclosed farmlands • Woodlands • Open waters (rivers, lochs, wetlands and floodplains) • Mountains • Moorland • Semi-natural grasslands • Urban • Population of c17,500 • 25.8% of population over 60 (higher than Scottish average) • Average health index in top 25% of Scotland (based on deprivation indices) • Extensive core paths network • 55 Munros including 5 summits over 4000 feet • 3 ski centres • National Cycle Network Route 7 • 1 Long Distance Route (Speyside Way) • Relatively low atmospheric pollution • Annual precipitation over 2250mm on summits and under 900mm in straths • Average annual snow cover 200 days on summits and 50 days on low-ground • Prevailing winds from south-west

Beech forest in the Central italian Apennines

The Hyytiälä SMEAR II site (Station for Measuring Ecosystem-Atmosphere Relations) is situated in the Hyytiälä Forestry Field Station of the University of Helsinki. The site consists is a managed, 60-yr old Scots pine forest stand. The main idea of SMEAR type infrastructures is continuous, comprehensive measurements of fluxes, storages and concentrations in the land ecosystem–atmosphere continuum. The forest measurements are operated continuously since 1996, and include e.g. leaf, stand and ecosystem scale measurements of greenhouse gases, volatile organic compounds, pollutants (e.g. O3, SO2, NOx) and aerosols, in addition to full suite of meteorological measurements. The biogeochemical cycles and vegetation–soil–atmosphere interactions are studied both experimentally and with long-term observations. The site has full carbon, water and nitrogen budgets made over 10 years, and it is a full ICOS ecosystem and atmospheric station. Ecosystem and atmosphere measurements also exist nearby in two open oligotrophic fen sites and a humic lake with forested catchment. Most of the measured data can be retrieved from: https://smear.avaa.csc.fi/